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human embryonic lung fibroblasts hfl 1  (Procell Inc)

 
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    Procell Inc human embryonic lung fibroblasts hfl 1
    Human Embryonic Lung Fibroblasts Hfl 1, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung fibroblasts hfl 1/product/Procell Inc
    Average 86 stars, based on 1 article reviews
    human embryonic lung fibroblasts hfl 1 - by Bioz Stars, 2026-06
    86/100 stars

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    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Hfl 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hfl 1 cells/product/ATCC
    Average 96 stars, based on 1 article reviews
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    		  Buy from Supplier
    hfl 1  (ATCC)
    96
    ATCC hfl 1
    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Hfl 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hfl 1/product/ATCC
    Average 96 stars, based on 1 article reviews
    hfl 1 - by Bioz Stars, 2026-06
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    human embryonic lung fibroblasts hfl 1  (Procell Inc)
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    Procell Inc human embryonic lung fibroblasts hfl 1
    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Human Embryonic Lung Fibroblasts Hfl 1, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung fibroblasts hfl 1/product/Procell Inc
    Average 86 stars, based on 1 article reviews
    human embryonic lung fibroblasts hfl 1 - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier
    hfl 1  (Procell Inc)
    86
    Procell Inc hfl 1
    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Hfl 1, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hfl 1/product/Procell Inc
    Average 86 stars, based on 1 article reviews
    hfl 1 - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier
    human riken hibepic human riken hfl  (ATCC)
    96
    ATCC human riken hibepic human riken hfl
    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Human Riken Hibepic Human Riken Hfl, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human riken hibepic human riken hfl/product/ATCC
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    human fetal lung fibroblasts hfl 1  (ATCC)
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    ATCC human fetal lung fibroblasts hfl 1
    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC <t>(blue),</t> <t>HFL-1</t> fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.
    Human Fetal Lung Fibroblasts Hfl 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fetal lung fibroblasts hfl 1/product/ATCC
    Average 96 stars, based on 1 article reviews
    human fetal lung fibroblasts hfl 1 - by Bioz Stars, 2026-06
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    		  Buy from Supplier

    Image Search Results


    Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.

    Journal: International Journal of Molecular Sciences

    Article Title: Proof-of-Concept Evaluation of Primary Human FAP-CAR-NK Cells Targeting Activated Fibroblasts in Pulmonary Fibrosis

    doi: 10.3390/ijms27094128

    Figure Lengend Snippet: Proof-of-concept activity of FAP-CAR-NK cells in a fibrotic human lung organoid co-culture model. ( A ) Bright-field images showing morphological alterations in lung organoid co-cultures under non-fibrotic control conditions (NC), profibrotic stimulation (PC), pirfenidone treatment (PFD), untransduced NK-cell treatment (NK), and FAP-CAR-NK-cell treatment (CAR-NK). Treatment-associated fragmented, debris-like material is particularly evident in the NK and CAR-NK groups. Images were acquired at 4× and 10× magnification. ( B ) Multichannel fluorescence imaging of the three-dimensional Matrigel microdroplet co-culture system showing lung organoids labeled with CellTracker Blue CMAC (blue), HFL-1 fibroblasts labeled with GFP (green), and effector cells labeled with CellTracker Deep Red (red); lower panels show magnified views of representative droplets (24 h). ( C ) RT-qPCR analysis of COL1A1, FAP, ACTA2, and SP-C expression in the indicated groups. qPCR primers are shown in . ( D ) Quantification of IFN-γ and TNF-α in culture supernatants after co-culture. Data are presented as mean ± SEM from three independently established organoid co-culture experiments ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant.

    Article Snippet: Phoenix-AMPHO and HFL-1 cells were obtained from the American Type Culture Collection (ATCC).

    Techniques: Activity Assay, Co-Culture Assay, Control, Fluorescence, Imaging, Labeling, Quantitative RT-PCR, Expressing